Asian Journal of Pharmaceutical Analysis

G. Krishnamoorthy ¹, C. Diana Priyadarshini ¹, R. Senthamarai ¹

Affiliations
1 Department of Pharmaceutical Analysis, Periyar College of Pharmaceutical Sciences, Tiruchirappalli – 620 021, IN

Abstract

A Simple, precise, rapid and accurate UV spectrophotometric method was developed for the estimation of Choline Bitartrate in bulk and its tablet formulation. UV scan of Choline Bitartrate showed max (absorbance) value at 208 nm in the concentration range of 10 μg/ml. This method is based on the measurement of light absorption in the UV region using water as solvent. Beer's Law plot was constructed by measuring the absorbance at 208 nm using various concentration of solution 5-35 μg/ml and show linearity and limit of detection. A simple and convenient method is applied to the determination of amount of choline bitartrate present in tablet formulation with high percentage of recovery, good accuracy and precision. The results are found satisfactory and reproducible. The proposed method was successfully extended to pharmaceutical preparation tablet.

Keywords

Choline Bitartrate, UV spectrophotometry Absorption Maxima, Pharmaceutical Formulation, Beer’s Range, Recovery studies.

Full Text

     

G. Krishnamoorthy ¹, N. Gayathri ¹, M. Gnanaraja ¹, R. Senthamarai ¹

Affiliations
1 Department of Pharmaceutical Analysis, Periyar College of Pharmaceutical Sciences, Tiruchirapalli – 620021, IN

Abstract

A simple spectrophotometric method has been developed for the determination of Lamivudine in bulk and solid dosage form. Lamivudine posses light absorption character in the UV region. The method is based on the measurement of light absorption in the UV region using distilled water. Lamivudine shows maximum absorbance at 271nm.The Beer's law obeyed in the concentration range of 10-60μg /ml. Results are found satisfactory and reproducible. The proposed method is validated with respect to accuracy, precision, linearity and by recovery studies. The method is applied successfully for the estimation of Lamivudine in tablet dosage form without the interference of excipients. The low value of standard deviation and coefficient of variation indicates that the proposed method is accurate, presise and reproducible.

Keywords

Analysis, Absorption Maxima, Beer's Range, Lamivudine Tablet, Recovery Studies.

Full Text